Synthesis, Physico-Chemical Characterization, and Cytotoxicity Assay of Mannich Base Derivatives with Heavy Metal Ions on RAW 264.7 Cell Line

Abstract

Heavy metal complexes of Pd (II), Pt(IV) and Au (III) with new ligands of mannich base S-(1-(benzothiazole-2-ylamino)methyl]-H-benzimidazole-2-yl]4-nitrobenzothioate as (L1), S-(1-((pyra- zine-2-carboxamido)methyl)-1-H-benzoimidaz-ole-2-yl)4-nitrobenzothioate as (L2) & S-(1-Morph- olinomethyl)-1-benzoimidazol-2-yl) 4-nitrobenzothioate as (L3) have been prepared and characterized in solid state, their most probable structure were established through a combination of different spectroscopic and analytical techniques such as: elemental analysis C.H.N.S, flame atomic absorption (A.A), FT-IR, UV–vis spectrophotometry, ¹H¹³CNMR, as well as conductivity and magnetic susceptibility measurements. The ratio of M:L and stability constant was determined by using molar ratio method in solution state. From the spectral studies, an octahedral geometry of PtL1, PtL2, AuL2, PtL3, PdL3 & AuL3 complexes, while PdL1, AuL1 & PdL2 have square planner geometry.  Cytotoxic effect of prepare ligands and their novel complexes were evaluated against RAW 264.7 cell line by using four different concentrations (400, 200, 100 & 50µg/ml) in exposure time 48 hrs. and compared this effect with control positive Cis-Pt.

Keywords: Mannich base, Benzimidazole, Analytical techniques, Cytotoxic, Exposure time.