The Effect of Ethanolic Extract of Mangosteen Peel As Adjuvant Therapy to TNF-Α And IL-10 in Wistar Rats Infected with M.tuberculosis H37rv

Abstract

Objective: This study was an experimental study of wistar rats infected with M. tuberculosis H37 Rv and then given isoniazid  INH as an anti-tuberculosis drug and ethanolic extract of mangosteen peel as adjuvant therapy. Method: Rats infected with M tuberculosis H37rv 10⁶ Mc Farland, as 100µl intratracheal. Rats were randomly divided into six groups control (K1), rats infected with M tuberculosis H37rv  only (K2), and rats infected with M tuberculosis H37rv and then treated with INH 100,200, 300 and 400 mg/kg body weight/ day (P1, P2, P3 and P4, respectivelyAfter administration of 4 weeks of therapy, the rats were sacrificed and the right lung  tissue was  collected to measured the levels of TNF α and IL 10 by using  ELISA. Results  The mean level of TNF α was found in the lowest P2 group compared to the treatment group (P1, P3 and P4) and the control was 24.16pg/ml. The  level of  IL 10 was found in the lowest P2 group compared to the treatment group (P1, P3 and P4) and the control was 232.75pg/ml. Ethanolic extract of mangosteen peel has a significant effect on decreasing levels of TNF α with a value of P = 0.000 <0.005 and IL 10 with a value of p = 0.001 <0.005.  Conclusion  Ethanolic extract of mangosteen peel is effective in reducing levels of TNF α and IL10 in adjuvant tuberculosis therapy, especially at doses of 200mg/kgbw/day, in smaller or larger doses there is an increased possibility of interaction between INH  and the active substances in the extract. Therefore further studies are needed to find the cause.

Keywords: M.tuberculosis, Mangosten, TNFα, IL10.