Impact of TGF B1 level and single nucleotide polymorphism in gall bladder inflammation

Abstract

The current study was conducted to investigate the association of TGF B1levels in serum and polymorphism in cholecystitis patients. The study include 100 blood samples collected from patient undergoing cholecystectomy at the general teaching Hilla Hospital from September 2016 to June 2017. Tgf-B1 concentration pointed significantly increase in the patients compared  with control group with mean value (82. 2pg/ml) for chronic cholecystitis patient  and (46.1pg/ml) for acute cholecystitis patient with   high significant  differences  between patient  and control. p=0.007. The results revealed increased level of TGF-B1 concentration in patient had cholesterol stone with value (65.33  pg/ml) followed pigment stone(60.11pg/ml) and mixed stone(27.62 pg/ml). Single nucleotide polymorphism of  TGF-B1 (-869 T/C showed high appearance of  TC heterozygous With (64) (75.2%)  than others genotypes ,  TT  and CC homozygous and  with (13)( 15%) (8)(9.4%) respectively .there is no significant differences in alleles frequency with severity of disease  while the genotype association of TGF-B1 appeared significant differences ( p=0.04, OR=4.56)  for acute infection in over dominate model compare with chronic ,cholesterol, pigmented and mixed stone patients  shows high appearance of  TC heterozygous  than other genotypes. 

Keywords : gallbladder , Polymorphism ,SNPs, TGF B1, Gallstone