Effects of Binahong (Anredera Cordifolia) Leaf Ethanol Extracts on Blood Glucose Levels and Pancreas Histopathology in Hyperglycemic Rats

Abstract

Background: The use of binahong leaf has been carried out for medical purposes for centuries. Several
studies have shown that binahong contain alkaloids, saponins, tannins, glycosides, and terpenes,
which can be known to have effects to reduce blood sugar levels. Objective: This study to determine
the effect of binahong leaf ethanol extract in reducing blood glucose and histopathological change in
hyperglycemic rats. Method: This study was an experimental study using Wistar rats that were
divided into five groups. The normal group was given with distilled water (P0), the positive control
group received glibenclamide (P1), and intervention group was given orally binahong leaf extract of
ethanol with a dose of 10mg/kg (P2), 15 mg/kg (P3) and 20 mg/kg (P4). Blood sugar level was
evaluated from the initial day of measurement to two weeks. Pancreas tissue was obtained to
evaluate the histopathological change. Results: Mean blood sugar level in each group respectively
94.28±6.19 mg/dL, 133.61±47.05 mg/dL, 117.56±26.73 mg/dL, 124.77±37.05 mg/dL, and 136.57±47.55
mg/dL. The result of Kruskal-Wallis test was p=0.011. Multiple comparison test showed significant
result between P4 with P0 (p=0.011). Conclusion: Binahong leaf ethanol extract with a dose of 20
mg/kg significantly reduce blood glucose level in the hyperglycemic rat.
Keywords: Binahong, Anredera cordifolia, Blood glucose, Hyperglycemic rat.