In-vitro Study of Layer Double Hydroxide Nanoparticles as Effective Carrier to Fight Breast Cancer Growth

Abstract

Vinblastine is well known alkaloid chemotherapy to treat various types of cancer including breast cancer, merging the field of nanotechnology and therapeutic drugs have brought a great interest. However nanodrugs delivery system has been given great attention in this field especially cancer-drug delivery system due to its significant properties which can be increased drug loading, reduced side effect of the drug and suppress cancer proliferation. Layers of magnesium and aluminum layered double hydroxide nanoparticles (LDH) have been synthesized by co-precipitation method with 25 to 50 nm size as SEM has revealed. Vinblastines have been intercalated in to LDH by ion exchange. Breast cancer cell line  MCF-7 have been  used to determine the cytotoxicity of Vin-NPs via MTT survival assay at different time interval 24 hrs, 48 hrs and 72 hrs. Cell viability of MCF-7 through MTT assay suggests drug intercalated LDHs has better drug delivery vehicle for cancer cell line against poor bioavailability of the pure Vinblastine. These observations clearly demonstrate that Vinblastine intercalated LDH NPs is a potential drug delivery vehicle for anticancer drugs which can increase drug efficiency , reduce the side effects and reduce the cost of the drug as well.

Keywords:  LDH nanoparticles, Vinblastine sulfate, MCF-7 cell line and MTT assay.