Protective Effect of Goat Kefir on Arsenic-Induced Ovarian Oxidative Stress in Female Rats
Abstract
Methods: twenty female wistar rats were randomly divided into five groups; one group was treated as control, one group was treated with arsenic 2 mg/kg/day only, while three groups were treated with different doses (1.25, 2.5, 5 mL/kg/day) of goat kefir and arsenic for 35 days. After 35 days, the rats were terminated, then the ovaries were taken for analysis of MDA levels and SOD activity using spectrophotometry.
Results: arsenic exposure significantly increased MDA levels but did not significantly decrease ovarian SOD activity compared to the control group. Kefir administration at doses of 1.25 and 2.5 mL/kg/day could reduce ovarian MDA levels although it was not statistically significant. However, MDA levels were greater in the 5 mL/kg/day group than the arsenic-only group. Kefir increased ovarian SOD activity at all three doses although it was not statistically significant.
Conclusion: goat kefir was able to reduce MDA levels and increase ovarian SOD activity in rats that were exposed to arsenic although it was not statistically significant.
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Flora SJ, Flora G, Saxena G. Arsenicals: toxicity, their use as chemical warfare agents, and possible remedial measures. InHandbook of Toxicology of Chemical Warfare Agents 2009;109-133.
Tseng CH. A review on environmental factors regulating arsenic methylation in humans. Toxicology and applied pharmacology. 2009;235(3):338-50.
Akram Z, Jalali S, Shami SA, Ahmad L, Batool S, Kalsoom O. Adverse effects of arsenic exposure on uterine function and structure in female rat. Experimental and Toxicologic Pathology. 2010;62(4):451-9.
Otite–Douglas MI, Fadairo EA. Studies of the Biochemical Effects of Arsenic Content of Carbide Induced Ripe Banana Fruit in the Rat. Oriental Journal of Chemistry. 2019;35(4):1332-7.
Silvestris E, Cohen M, Menezo Y. Oxidative stress (OS) and DNA methylation errors in reproduction a place for a support of the one carbon cycle (1-C Cycle) before conception. of. 2016;8:8-12.
Golub MS, Hogrefe CE, Germann SL, Lasley BL, Natarajan K, Tarantal AF. Effects of exogenous estrogenic agents on pubertal growth and reproductive system maturation in female rhesus monkeys. Toxicological Sciences. 2003;74(1):103-13.
Sharma B, Singh S, Siddiqi NJ. Biomedical implications of heavy metals induced imbalances in redox systems. BioMed research international. 2014.
Susko ML, Bloom MS, Neamtiu IA, Appleton AA, Surdu S, Pop C, Fitzgerald EF, Anastasiu D, Gurzau ES. Low-level arsenic exposure via drinking water consumption and female fecundity-A preliminary investigation. Environmental research. 2017;154:120-5.
Flora SJ. Arsenic-induced oxidative stress and its reversibility. Free Radical Biology and Medicine. 2011;51(2):257-81.
Ronchetti SA, Bianchi MS, Duvilanski BH, Cabilla JP. In vivo and in vitro arsenic exposition induces oxidative stress in anterior pituitary gland. International journal of toxicology. 2016;35(4):463-75.
Rao CV, Pal S, Mohammed A, Farooqui M, Doescher MP, Asch AS, Yamada HY. Biological effects and epidemiological consequences of arsenic exposure, and reagents that can ameliorate arsenic damage in vivo. Oncotarget. 2017;8(34):57605.
Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. British journal of pharmacology. 2004;142(2):231-55.
Qian Y, Castranova V, Shi X. New perspectives in arsenic-induced cell signal transduction. Journal of inorganic biochemistry. 2003 Aug 1;96(2-3):271-8.
Mehta M, Hundal SS. Effect of sodium arsenite on reproductive organs of female Wistar rats. Archives of environmental & occupational health. 2016;71(1):16-25.
Farnworth ER. Kefir–a complex probiotic. Food Science and Technology Bulletin: Fu. 2006;2(1):1-7.
Chen Z, Shi J, Yang X, Nan B, Liu Y, Wang Z. Chemical and physical characteristics and antioxidant activities of the exopolysaccharide produced by Tibetan kefir grains during milk fermentation. International Dairy Journal. 2015;43:15-21.
Prado MR, Blandón LM, Vandenberghe LP, Rodrigues C, Castro GR, Thomaz-Soccol V, Soccol CR. Milk kefir: composition, microbial cultures, biological activities, and related products. Frontiers in microbiology. 2015;6:1177.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95(2):351-8.
Mondal S, Mukherjee S, Chaudhuri K, Kabir SN, Kumar Mukhopadhyay P. Prevention of arsenic-mediated reproductive toxicity in adult female rats by high protein diet. Pharmaceutical biology. 2013;51(11):1363-71.
De Vizcaya-Ruiz A, Barbier O, Ruiz-Ramos R, Cebrian ME. Biomarkers of oxidative stress and damage in human populations exposed to arsenic. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2009;674(1-2):85-92.
Wang S, He G, Chen M, Zuo T, Xu W, Liu X. The role of antioxidant enzymes in the ovaries. Oxidative medicine and cellular longevity. 2017.
Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford University Press, USA; 2015.
Cooper KL, Liu KJ, Hudson LG. Enhanced ROS production and redox signaling with combined arsenite and UVA exposure: contribution of NADPH oxidase. Free Radical Biology and Medicine. 2009;47(4):381-8.
Winarsi H. Antioksidan alami dan radikal. Kanisius; 2005.
Seyidoglu N, Aydin C. Stress, Natural Antioxidants and Future Perspectives. InThe Health Benefits of Foods-Current Knowledge and Further Development 2020. IntechOpen.
Fiorda FA, de Melo Pereira GV, Thomaz-Soccol V, Medeiros AP, Rakshit SK, Soccol CR. Development of kefir-based probiotic beverages with DNA protection and antioxidant activities using soybean hydrolyzed extract, colostrum and honey. LWT-Food Science and Technology. 2016;68:690-7.
Noori S. An overview of oxidative stress and antioxidant defensive system. Open access scientific reports. 2012;1(8):1-9.
Kunaepah U. Pengaruh Lama Fermentasi Dan Konsentrasi Glukosa Terhadap Aktivitas Antibakteri, Polifenol Total Dan Mutu Kimia Kefir Susu Kacang Merah The Effect Of Fermentation Duration And Glucose Concentration On Antibacterial Activity, Total Polyphenol And Chemical Quality Of Kidney Bean Milk Kefir (Doctoral dissertation, Program Pasca Sarjana Universitas Diponegoro).
Radhouani H, Gonçalves C, Maia FR, Oliveira JM, Reis RL. Kefiran biopolymer: Evaluation of its physicochemical and biological properties. Journal of Bioactive and Compatible Polymers. 2018;33(5):461-78.
Radhouani H, Gonçalves C, Maia FR, Oliveira JM, Reis RL. Biological performance of a promising Kefiran-biopolymer with potential in regenerative medicine applications: A comparative study with hyaluronic acid. Journal of Materials Science: Materials in Medicine. 2018;29(8):124.
Wang Y, Yang Z, Wei X. Antioxidant activities potential of tea polysaccharide fractions obtained by ultra filtration. International journal of biological macromolecules. 2012;50(3):558-64.
Liu SH, Sun SW, Tian ZF, Wu JY, Li XL, Xu CP. Antioxidant and hypoglycemic activities of exopolysaccharide by submerged culture of inocutus hispidus. Indian journal of pharmaceutical sciences. 2015;77(3):361.
Liu JR, Chen MJ, Lin CW. Antimutagenic and antioxidant properties of milk− kefir and soymilk− kefir. Journal of agricultural and food chemistry. 2005 Apr 6;53(7):2467-74.
Bouayed J, Bohn T. Exogenous antioxidants—double-edged swords in cellular redox state: health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative medicine and cellular longevity. 2010;3(4):228-37.
Martin KR, Barrett JC. Reactive oxygen species as double-edged swords in cellular processes: low-dose cell signaling versus high-dose toxicity. Human & experimental toxicology. 2002;21(2):71-5.
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