Role of PPAR-Α Signaling Pathway on the Protection of Antituberculosis-Induced Liver Damage by Purple Sweet Potato Extract

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Abstract

Background: Peroxisome proliferator activated receptor-α (PPAR-α) participates in protecting the liver from many cases of liver damage. It is involved in reducing lipid accumulation, oxidative stress and inflammation. Purple sweet potato extract is one of herbal extract that posses hepatoprotector activity related to its antioxidant and antiinflammatory action. Its hepatoprotective effect on isoniazid and rifampicin induced hepatic injury has not been established yet, as well as its effect on PPAR-α expression and activation in the liver. The aim of this research was to investigate the hepatoprotective effect of purple sweet potato extract on hepatic injury induced by isoniazid and rifampicin, as well as the effect of purple sweet potato on PPAR-α expression and activation in the liver.
Methods: The study design was randomized post test only control group design. We included male Wistar rats, age 8-12 weeks, weight 180-220 gram (divided into 3 groups: control, intervention and normal group). The extraction was performed with masseration technique. PPAR-α expression was measured with ELISA; activated PPAR-α was detected using immunohistochemistry; ALT level was measured with spectrophotometry. Data were analyzed using One Way Anova test, continued with Post Hoc test.
Results: The result represented that PPAR-α expression in intervention group was significantly higher than control (1.344±0.249 vs. 0.587±0.306; p<0.001). The activated PPAR-α expression in intervention group was also significantly higher than control (2.736±0.628 vs. 1.903±0.397; p<0.001). ALT level in intervention group was significantly lower than control (24.169±1.080 vs. 39.272±0.513; p<0.001).
Conclusion: In conclusions, hepatic damage induced by INH-RIH could be prevented by purple sweet potato ethanolic extract administration which potentially improved liver function through increased PPAR-α expression and activation.

Keywords: antituberculosis; hepatoprotective; ppar; purple sweet potato

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References

Neels JG and Grimaldi PA (2014) Physiological functions of peroxisome proliferator-activated receptor β. Physiology Review, 94: 795-858.

Nan Y-M, Wang R-Q, Fu N (2014) Peroxisome proliferator activated receptor α, a potential therapeutic target for alcoholic liver disease. World Journal of Gastroenterology, 20(25): 8055-8060.

Grygiel-Gorniak B (2014) Peroxisome proliferator-activated receptors and their ligands: nutritional and clinical implications: a review. Nutrition Journal, 13(17): 1-10.

Hassan HM, Guo H, Yousef BA, Guerram M, Hamdi AM, Zhang L, Jiang Z (2016) Role of inflammatory and oxidative stress, cytochrome p450 2e1 and bile acid disturbance in rat liver injury induced by isoniazid and lipopolysaccharide cotreatment. Antimicrobial Agents and Chemotherapy, 60(9): 5285-5293.

Sozanski T, Kucharska AZ, Rapak A, Szummy D, Trocha M, Merwid-Lad A, et al (2016) Iridoid-loganic acid versus anthocyanins from the Cornus mas fruits (corneelian cherry): common and different effects on diet-induced atherosclerosis, PPARs expression and inflammation. Atherosclerosis, 254: 151-160.

Mi W, Han F, Liang J, Liang Y, Guan B, Xu H (2018) Purple sweet potato anthocyanins attenuates steatohepatitis induced by high fat diet combined with carbon tetrachloride in rats. Wei Sheng Yan Jiu, 47(4): 517-524.

Miguel MG (2011) Anthocyanins: antioxidant and or antiinflammatory activities. Journal of Applied Pharmaceutical Science, 1(6): 7-15.

Artini IGA, Jawi IM, Astawa INM, Sutirta-Yasa IWP (2020) Developing animal model for analysing liver toxicity of isoniazid and rifampicin combination. Journal of Global Pharma Technology, 12(1): 391-396.

Pawlak M, Lefebvre P, Staels B (2015) Molecular mechanism of PPARα action and its impact on lipid metabolism, inflammation and fibrosis in non-alcoholic fatty liver disease. Journal of Hepatology, 62: 720-733.

Brunmeir R and Xu F (2018) Functional regulation of PPARs through post-translational modifications. International Journal of Molecular Sciences, 19(1738): 1-16.

Iwamoto F, Umemoto T, Motojima K, Fujiki Y (2011) Nuclear transport of peroxisome proliferator activated receptor α. The Journal of Biochemistry, 149(3): 311-319.

Esposito E, Cuzzocrea S, Meli R (2006) Peroxisome proliferator-activated receptors and shock state. The Scientific World Journal, 6: 1770-1782.

Farrell NJ, Norris GH, Ryan J, Porter CM, Jiang C, Blesso CN (2015) Black elderberry etract attenuates inflammation and metabolic dysfunction in diet-induced obese mice. British Journal of Nutrition, 114: 1123-1131.

Kim T and Yang Q (2013) Peroxisome proliferator-activated receptors regulate redox signaling in the cardiovascular system. World Journal of Cardiology, 5(6): 164-174.

Qiu L-X and Chen T (2015) Novel insights into the mechanisms whereby isoflavones protect against fatty liver disease. World Journal of Gastroenterology, 21(4): 1099-1107.

Yan F, Chen Y, Azat R, Zheng X (2017) Mulberry anthocyanin extract ameliorates oxidative damage in hepG2 cells and prolongs the lifespan of caenorhabditis elegans through mapk and nrf2 pathways. Oxidative Medicine and Cellular Longevity, 2017: 1-12.

Jang H, Park M, Kim H, Lee Y, Hwang K, Park J, et al (2012) Black rice (oryza sativa l.) extract attenuates hepatic steatosis in c57bl/6j mice fed a high-fat diet via fatty acid oxidation. Nutrition and Metabolism, 9(27): 1-11.

Felix ADR, Takahashi N, Takahashi M, Katsumata-Tsuboi R, Satoh R, Hui TS, et al (2017) Extract of black and brown rice powders improve hepatic lipid accumulation via the activation of pparα inobese and diabetic model mice. Bioscience, Biotechnology, and Biochemistry, 81(11): 2209-2211.

Eslamparast T, Eghtesad S, Poustchi H, Hekmatdoost A (2015). Recent advances in dietary supplementation, in treating non-alcoholic fatty liver disease. World Journal of Hepatology, 7(2): 204-212.

Medjakovic S, Mueller M, Jungbauer A (2010) Potential health-modulating effects of isoflavones and metabolites via activation of ppar and ahr. Nutrients, 2: 241-279.

Dominguez-Avila JA, Gonzalez-Aguilar GA, Alvarez-Parrilla E, de la Rosa LA (2016) Modulation of ppar expression and activity in response to polyphenolic compounds in high fat diets. International Journal of Molecular Sciences, 17: 1002-1018.

Pall ML and Levine S (2015) Nrf2, a master regulator of detoxification and also antioxidant, antiinflammatory and other cytoprotective mechanisms, is raised by health promoting factors. Acta Physiologica Sinica, 67(1): 1-18.

Lee Y, Yoon Y, Yoon H, Park H, Song S, Yeum K (2017) Dietary anthocyanins against obesity and inflammation. Nutrients, 9: 1089-1104.

Yang L, Xian D, Xiong X, Lai R, Song J, Zhong J (2018) Proanthocyanidins against oxidative stress: from molecular mechanisms to clinical applications. Biomed Research International, 2018: 1-11.

Vendrame S and Klimis-Zacas D (2015) Antiinflammatory effect of anthocyanins via modulation of nuclear factor-κB and mitogen-activated protein kinase signaling cascades. Nutrition Reviews, 73(6): 348-358.

Korbecki J, Bobinski R, Dutka M (2019) Self-regulation of the inflammatory response by peroxisome proliferator-activated receptors. Inflammation Research, 68(6): 443-458.

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