Endophytic Fungi from Piper retrofractum VAHL: Isolation, Phytochemical Analysis, Antibacterial and Antioxidant Activities

Dwi Koko Pratoko

Abstract

Objective: Piper retrofractum Vahl. is an Indonesian plant that has several activities such as antibacterial, antifungal, antioxidant, etc. Endophytic fungi are fungal microbe which can produce secondary metabolites that associated with medical plants can be exploited for curing many diseases. This is the first study of endophytic fungi from P. retrofractum which can be an alternative source of natural product besides its host, P. retrofractum. Seven endophytic fungi were isolated from the fruits and leaves of P. Retrofractum and investigated for their antibacterial and antioxidant activities.

Methods: All the isolated fungi were tested for their antibacterial activity using disc diffusion method with several series of concentrations and antioxidant capacity using the DPPH method.  Result: The highest activity both antibacterial and antioxidant was demonstrated by DPR-1 extract against Staphylococcus aureus and Pseudomonas aeruginosa. This extract contained alkaloids, steroids, and flavonoid compounds.

Conclusion: This study reported that endophytic fungi from P. retrofractum indicate as a potential source of bioactive and chemically novel compounds.

Keywords: Piper retrofractum, endophytic fungi, antibacterial activity, antioxidant activity

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References

P. Warrier, V. Nambiar, and K. Raman, Piper longum Linn.: Indian medicinal plants. India.: Orient Longman Ltd. Madrs, 1995.

I. P. G. Krisnawan, P. A. Sandhi, and A. S. Duniaji, “Daya hambat ekstrak daun cabe jawa (Piper retrofactum Vahl.) terhadap pertumbuhan Staphylococcus aureus,†vol. 6, no. 2, pp. 1–10, 2017.

E. R. Sari and E. R. Nugraheni, “Antifungal activity test of Piper retrofractum leaf ethanol extract on Candida albicans growth,†Biofarmasi, vol. 11, no. 2, pp. 36–42, 2013.

Y. Jamal, P. Irawati, A. Fathoni, and A. Agusta, “Chemical constituents and antibacterial effect of essential oil of Javanese pepper leaves (Piper retrofractum Vahl.),†Indones. Inst. Sci. Res. Cent. Biol. LIPI, Bogor, vol. 23, pp. 65–72, 2013.

N. J. Reddy, D. N. Vali, M. Rani, and S. S. Rani, “Evaluation of antioxidant , antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit,†vol. 34, pp. 115–122, 2014.

M. Khan and M. Siddiqui, “Antimicrobial activity of piper fruits,†Nat Prod Rad, vol. 6, no. 111–113, 2007.

A. A. L. Gunatilaka, “Natural products from plant-associated microorganism: Distribution, structural diversity, bioactivity and implications of their occurrence,†J. Nat. Prod, vol. 69, pp. 509–526, 2006.

J. S. Bhore and G. Sathisha, “Screening of endophytic colonizing bacteria for cytokinin-like compounds : Crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay,†World J. Agric. Sci., vol. 6, no. 4, pp. 345–352, 2010.

G. Strobel and B. Daisy, “Bioprospecting for microbial endophytes and their natural products,†Microb. Mol Biol Rev 67, vol. 4, pp. 491–502, 2003.

B. D. Rekha and M. B. Shivanna, “Diversity, antimicrobial and antioxidant activities of fungal endophytes in Cynodon dactylon (L.) Pers. and Dactyloctenium aegyptium (L.) P.,†Int. J. Curr. Microbiol. Appl. Sci., vol. 3, pp. 573–591, 2014.

M. Stinson, D. Ezra, W. Hess, J. Sears, and G. Strobel, “An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compounds,†Plant Sci, vol. 165, pp. 913–922, 2003.

M. Corrado and K. Rodrigues, “Antimicrobial evaluation of fungal extracts produced by endophytic strains of Phomopsis sp.,†J Basic Microbiol, vol. 44, pp. 157–160, 2004.

D. Ezra, W. Hess, and G. Strobel, “New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus.,†Microbiology, vol. 150, pp. 4023–4031, 2004.

Y. Huang, J. Wang, G. Li, Z. Zheng, and W. Su, “Antitumor and antifungal activities in endophyticfungi isolated from pharmaceutical plants,†Immunol. Med. Microbiol., vol. 31, pp. 163–167, 2001.

K. Selim, E.-B. AA, A.-R. TM, and El-Diwany, “Biology of endophytic fungi,†Curr. Res. Environ. Appl. Mycol., vol. 2, no. 1, pp. 31–82, 2012.

S. Wiyakrutta, N. Sriubolmas, W. Panphut, N. Thongon, K. D. Kanjana, and N. Ruangrungsi, “Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants,†World J. Microbiol. Biotechnol., vol. 20, pp. 265–272, 2004.

S. J. Higginbotham, A. E. Arnold, A. Ibanez, C. Spadafora, P. D. Coley, and T. A. Kursar, “Bioactivity of fungal endophytes as a function of endophyte taxonomy and the taxonomy and distribution of their host plants,†PLoS One, vol. 8, p. 73192, 2013.

S. Kim, D.-S. Shin, T. Lee, and K.-B. Oh, “Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity,†J Nat Prod 67, pp. 448–450, 2004.

Y. Li, Y. Song, J. Liu, Y. Ma, and R. Tan, “Anti-Helicobacter pylori substances from endophytic fungal cultures,†World J Microbiol Biotechnol 21, pp. 553–558, 2005.

J. Liu et al., “Aspergillus fumigatus CY018, an endophytic fungus in Cynodon dactylon as a versatile producer of new and bioactive metabolites,†J Biotechnol 114, pp. 279–287, 2004.

P. Chomchoen, S. Wiyakrutta, N. Sriubolmas, N. Ngamrojanavanich, D. Isarangkul, and P. Kittakoop, “3-Nitropropionic acid (3-NPA), a potent antimycobacterial agent from endophytic fungi: is 3-NPA in some plants produced by endophytes?,†J Nat Prod 68, pp. 1103–1105, 2005.

I. Atmosukarto, U. Castillo, W. Hess, J. Sears, and G. Strobel, “Isolation and characterization of Muscodor albus I-41.3 s, a volatile antibiotic producing fungus,†Plant Sci 169, pp. 854–861, 2005.

U. Purwanto, F. Pasaribu, and M. Bintang, “Isolasi bakteri endofit dari tanaman sirih hijau (Piper betle L.) dan potensinya sebagai penghasil senyawa antibakteri,†Curr. Biochem., vol. 1, no. 1, pp. 51–57, 2014.

F. Uzma and S. Chowdappa, “Antimicrobial and antioxidant potential of endophytic fungi isolated from ethnomedicinal plants of Western Ghats, Karnataka,†J. Pure Appl. Microbiol., vol. 11, no. 2, 2017.

S. Feitosa et al., “Antimicrobial activity of crude extracts of endophytic fungi from Oryctanthus alveolatus ( Kunth ) Kuijt ( Mistletoe ),†African J. Microbiol. Res., vol. 12, no. 11, pp. 263–268, 2018.

A. S. Nugraha et al., “Antibacterial and anticancer activities of nine lichens of Indonesian Java island,†J. Biol. Act. Prod. from Nat., vol. 9, no. 1, pp. 39–46, 2019.

H. Wagner and S. Bladt, Plant Drug Analysis: A thin layer chromatography atlas, 2nd ed. Berlin: Springer-Verlag, 1996.

D. K. Pratoko, F. A. Wardhani, N. Kristiningrum, F. A. Fajrin, and D. A. Pangaribowo, “Kadar fenolat dan flavonoid total serta kapasitas antioksidan ekstrak etanol dan fraksi jahe merah (Zingiber officinale var. Rubrum),†Al-Kimia, vol. 6, no. 2, pp. 166–177, 2018.

H. Y. Lai, Y. Y. Yau, and K. H. Kim, “Blechnum orientale Linn-a fern with potential as antioxidant, anticancer and antibacterial agent,†BMC Complem. Altern. M., vol. 10, no. 15, 2010.

R. H. Patil, M. P. Patil, and V. L. Maheshwari, Bioactive secondary metabolites from endophytic fungi: A review of biotechnological production and their potential applications, 1st ed. 2016.

L. Panawala, “difference between gram positive and gram negative bacteria difference between gram positive and gram negative bacteria stunning images of cells discover how scientists use main difference – gram positive vs gram negative bacteria,†Biology (Basel)., pp. 1–13, 2017.

D. Daniels, B. Biswas, K. Rogers, F. Mclaughlin, D. Daniels, and A. Yadav, “Antimicrobial activities of leaf extracts of guava ( Psidium guajava L .) on two gram- negative and gram-positive bacteria A,†Int. J. Microbiol., vol. 2013, pp. 1–8, 2015.

L. Hermawan, Purwanti, and U. A. Dasuki, “Identifikasi senyawa flavonoid dari daun pakis sayur [Diplazium esculentum (retz.) swartz],†pp. 642–650, 2017.

X. Liu, M. Dong, X. Chen, M. Jiang, X. Lv, and G. Yan, “Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba,†Food Chem., vol. 105, pp. 548–554, 2007.

A. N. Panche, A. D. Diwan, and S. R. Chandra, “Flavonoids: an overview,†J. Nutr. Sci., vol. 5, no. 47, pp. 1–15, 2016.

I. Y. Ningsih, S. Zulaikhah, M. A. Hidayat, and B. Kuswandi, “Antioxidant activity of various kenitu (Chrysophyllum Cainito L.) leaves extracts from Jember, Indonesia,†Agric. Agric. Sci. Procedia, vol. 9, no. December, pp. 378–385, 2016.

M. Antony, D. Menon, J. James, L. Dev, K. Arun, and V. Thankaman i, “Phytochemical analysis and antioxidant activity of alstonia scholaris,†Pharmacogn. J., vol. 3, no. 26, pp. 13–18, 2011.

N. Al-Jaber, A. Awaad, and J. Moses, “Review on some antioxidant plants growing in Arab world,†J. Saudi Chem. Soc., vol. 4, no. 15, pp. 293–307, 2011.

T. T. Cushnie, B. Cushnie, and A. J. Lamb, “Alkaloids: An overview of their antibacterial, antibiotic-enhanching and antivirulence activities,†Int. J. Antimicrob. Agents, vol. 44, no. 5, pp. 377–386, 2014.

D. Mabhiza, T. Chitemerere, and S. Mukanganyama, “Antibacterial properties of alkaloid extracts from Callistemon adoensis against Staphylococcus aureus and Pseudomonas aeruginosa,†Int. J. Med. Chem., 2016.

L. Othman, A. Sleiman, R. M. Abdel-massih, and M. Sciences, “Antimicrobial activity of polyphenols and alkaloids in Middle Eastern plants,†Front. Microbiol., 2019.

D. T. Wicklow, S. Roth, S. T. Deyrup, and J. B. Gloer, “A protective endophyte of maize: Acremonium zeae antibiotics inhibitory to Aspergillus flavus and Fusarium verticilioides,†Mycol. Res., vol. 109, no. 5, pp. 610–618, 2005.

A. Dalimunthe, A. P. Z. Hasibuan, J. Silalahi, S. F. Sinaga, and D. Satria, “Antioxidant activity of alkaloid compounds from Litsea cubeba Lour.,†Orient. J. Chem., vol. 32, no. 2, pp. 1149–1152, 2018.

S. Sivakrishnan and M. Swamivelmanickam, “Journal of Global Pharma Technology Antioxidant Potential , Total Phenolic and flavonoids content of aerial parts of ethanolic extract of Cordia obliqua,†J. Glob. Pharma Technol., vol. 11, no. 8, pp. 5–7, 2019.

J. K. Harper, A. M. Arif, E. J. Ford, G. A. Strobel, J. A. Porco, and D. P. Tomer, “Pestacin: a 1, 3-dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities,†Tetrahedron, vol. 59, p. 2471_2476, 2003.

T. T. Cushnie and A. J. Lamb, “Antimicrobial activity of flavonoids,†Int. J. Antimicrob. Agents, vol. 26, no. 5, pp. 343–356, 2015.

J. Zhang, D. Liu, H. Wang, T. Liu, and Z. Xin, “Fusartricin, a sesquiterpenoid ether produced by an endophytic fungus Fusarium tricinctum Salicorn 19,†Eur. Food Res. Technol., vol. 240, pp. 805–814, 2015.

E. Elfita, M. Muharni, and M. M. Rizki, “Isolation of antioxidant compound from endophytic fungi Acremonium sp. from the twigs of Kandis Gajah, Makara,†J. Sci, vol. 16, pp. 46–50, 2012.

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