Extracellular Biosynthesis of TiO2 Nanoparticles Using Supernatant Culture of Lactobacillus crispatus

Abstract

In this study, titanium dioxide nanoparticles were synthesized biologically using isolated L.crispatus from vagina of healthy women. The formation of TiO₂ nanoparticles was characterized by atomic force microscope (AFM) using supernatant culture of L.crispatus .Extracellular biosynthesized TiO₂ nanoparticles occurred with the average size (87.88) nm. For further characterization, the x-ray diffraction (XRD), energy dispersive x –ray spectroscopy (EDX), and scanning electron microscope (SEM) were used. XRD Confirmed the formation of crystallographic plane of anatase of TiO₂ nanoparticles indicating that nanoparticles corresponded to anatase crystalline, while the EDX analysis of TiO₂ nanoparticles indicated that the synthesized nanoparticles were anatase form and not frutile or brookite form. The SEM images of the synthesized TiO₂ nanoparticles were showed spherical or oval in shape. The effect of independent variables, including different incubation times and storage condition on the biosynthesis of TiO₂ nanoparticles was investigated. The average size (70.98)nm of TiO₂ nanoparticle was observed after 72 h .The stability of the synthesized TiO₂ nanoparticles for (1,2,3)months under cooling condition was studied and the result revealed that the synthesized TiO₂ nanoparticle remained stable without change in color even after storage for three months under cooling conditions.

Keywords: TiO₂ nanoparticles; Biosynthesis; Lactobacillus Crispatus; Incubation time; Storage time.