Determain Kind and Concentration of Allium sativum L.Plant Ingredients and its Effect on Isolated Bacteria Causing Urinary Tract Infections

Abstract

Atotal of  (190)  samples urinary were collected patients with Urinary Tract Infections(UTIs) from the period 2017/11/18 to 2018/2/17 ,for determain kind and concentration of Allium sativum L.ingredients and its effect on growth of the Gram-negative bacteria isolated from urinary tract infections in Al-Alam city/Tikrit Governoratr/Iraq,The study was carried out in the laboratories of Al-Alam Hospital and the laboratories of the College of Education for Women , Department of life Science,chemical analysis by HPLC apparatua showed the plant contain severel active ingrdients: S-ally cysteine,Y-glutomycysteine, Vinyl-{4H}1,2dithlin(agoene),di-allyldisulfide and Diallytrisulfide with concentrate,5.72% 9.75%, 53.09%,9.72%,12..85% and 10.60% respectively,also appeared many bacteria genus on In the samples taken from patients patients: E.coli, Klebsiella, Pseudomonus,Proteus,Serratia and Chromobacterium with isolates concentration reached for each genus reached, 44.2%, 23.5%, 17.6%, 5.5%, 5.8% and 2.9% respectively,and the current study for aquoues and alcoholic for  extracts appeared a clear contrast on inhibition of studied bacteria kinds, as it was alcoholic extract of Allium sativum more inhibiton than aquoues extract on the concentration 100% throught observation inhibition diameter on the concentration 25,50 and 100%.

Keywords: Allium sativum+plant extract+bacteria.