Morus alba and Morus rubra alleviate hepatic disorder, oxidative DNA damage and gene expression profile change in obese rats
Abstract
Objective: To examine the impact of Morus rubra and Morus alba extracts on physiological and metabolic functions of obese male rats induced by high fat diet. Methods: Orlistat was used as standard drug. Seventy Wistar male rats (weighing 150-160 g) were fed for 12 weeks with high fat diet to induce obesity. The obese rats were randomly assigned to three groups (10 rats each) and treated with Morus rubra and Morus alba extracts or orlistat for 6 consecutive weeks. The level of visfatin was measured by enzyme-linked-immunosorbent. The suppression impact of extracts against DNA adducts 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 2-deoxyguanosine (2-dG) generation was measured by high-performance liquid chromatography. Expression of genes including ATP- citrate-lyase, peroxisome-proliferator-activated receptor-γ and HMG CoA reductase in liver tissues were also measured by quantitative real time-polymerase chain reaction. Results: Treatment with the Morus rubra and Morus alba extracts and orlistat significantly decreased visfatin level. Morus rubra and Morus alba extracts significantly decreased the levels of mRNA expression of ATP-citrate-lyase, HMG CoA reductase genes, and peroxisome-proliferator-activated receptor-γ compared with high fat diet rats. In addition, Morus rubra and Morus alba extracts decreased the level of 8-OHdG/2-dG ratio. Conclusions: The ameliorating effect of Morus rubra and Morus alba extracts on visfatin levels reducing fat may be attributed to the rich content of polyphenolic which suppresses lipid synthesis in liver tissues. Moreover, anti-DNA damage effect of Morus rubra and Morus alba extracts suggests their role as natural antioxidants against diseases such as obesity associated with genetic damage.
Full Text:
PDFReferences
References
Smith BW, Adams LA (2011) Nonalcoholic fatty liver disease and diabetes mellitus: pathogenesis and treatment. Nat. Rev. Endocrinol., 7(8): 456-465.
Rodrigues EL, Marcelino G, Silva GT, Figueiredo PS, Garcez WS, Corsino J, et al. (2019) Nutraceutical and medicinal potential of the Morus species in metabolic dysfunctions. Int. J. Mol. Sci., 20 (2): E301.
Jang HH, Nam SY, Kim MJ, Kim JB, Choi JS, Kim HR, (2017) Agrimonia pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response. BMC Complement. Altern. Med., 17 (1): 442.
Memmert S, Damanaki A, Nokhbehsaim M, Nogueira AV, Eick S, Cirelli JA, et al. (2019) Regulation of somatostatin receptor 2 by proinflammatory, microbial and obesity-related signals in periodontal cells and tissues. Head Face Med.15 (1): 2.
Ozgocmen M, Gokcimen A, Oncu M, Akdogan M, Bayram D, Armagan I (2018) Effects of non-alcoholic fatty liver disease on visfatin and IL-6 levels in mice: an immunohistochemical study. Immunochem. Immunopathol., 4: 131.
Heyman-Lindén L, Seki Y, Storm P, Jones HA, Charron MJ, Berger K, et al (2016) Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet. J. Nutr. Biochem. 27: 79-95.
van Breda SGJ, Briedé JJ, de Kok TMCM (2018) Improved preventive effects of combined bioactive compounds present in different blueberry varieties as compared to single phytochemicals. Nutrients, 11 (1): E61.
Adaramoye OA, Akintayo O, Achem J, Fafunso MA (2008) Lipid-lowering effects of methanolic extract of Vernonia amygdalina leaves in rats fed on high cholesterol diet. Vasc. Health Risk Manag., 4 (1): 235-241.
El-Baz F, Aly HF, Abd-Alla HI, Fayed DB (2018) Therapeutic impact of berries (Morus alba and Morus rubra) fruits extract in the regression of high fat diet induced cardiac dysfunction in rats. Asian J. Pharm. Clin. Res., 11: 314-320.
Hwang YJ, Lee EJ, Kim HR, Hwang KA (2013) In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var. Lilacina. BMC Complement. Altern. Med., 13: 310.
Shalaby HMA, Tawfek NS, Abo-El Hussein BK, Abd El-Ghany MSM (2015) The assessment of some biochemical and immunological effects by amphetamine and orlistat on obesity in rats. Food Public Health, 4: 185-192.
Shalaby NM, Abd-Alla HI, Aly HF, Albalawy MA, Shaker KH, Bouajila J (2014) Preliminary in vitro and in vivo evaluation of antidiabetic activity of Ducrosia anethifolia Boiss. and its linear furanocoumarins. Biomed. Res. Int., 2014: 480545 -480558.
Patel M, Liang LP, Roberts Ii LJ (2001) Enhanced hippocampal F2‐isoprostane formation following kainate‐induced seizures. J. Neurochem., 79(5): 1065-1069.
El-Baz FK, Khalil WK, Booles HF, Aly HF, Ali GH (2016) Dunaliella salina suppress oxidative stress, alterations in the expression of pro-apoptosis and inflammation related genes induced by STZ in diabetic rats. Int. J. Pharm. Sci. Rev. Res., 38: 219-226.
Zhang W, Zhao D, Meng Z, Wang H, Zhao K, Feng X, Li Y (2018) Association between circulating visfatin and gestational diabetes mellitus: A systematic review and meta-analysis. Acta Diabetol., 55(11): 1113-1120.
He H, Lu YH (2013) Comparison of inhibitory activities and mechanisms of five mulberry plant bioactive components against α-glucosidase. J. Agric. Food Chem., 61: 8110-8119.
El-Baz FK, Hassan AZ, Abd-Alla HI, Aly HF, Mahmoud K (2017) Phytochemical analysis, assessment of antiproliferative and free radical scavenging activity of Morus alba and Morus rubra fruits. Asian J. Pharm. Clin. Res., 10: 189-199.
Mohamed NZ, Abd-Alla HI, Aly HF, Mantawy M, Ibrahim N, Hassan SA (2014) CCl4-induced hepatonephrotoxicity: protective effect of nutraceuticals on inflammatory factors and antioxidative status in rat. J. Appl. Pharm. Sci., 4: 87-100.
El-Baz FK, Aly HF, Abd-Alla HI, Ali SA (2018) Neurorestorative mulberries potential of Alzheimer's disease in animal model. Asian J. Pharm. Clin. Res., 11: 318-324.
Katsube T, Yamasaki M, Shiwaku K, IshijimaT, Matsumoto I, Abec K, et al. (2010) Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. J. Sci. Food Agric., 90: 2386–2392.
El-Baz F, Aly HF, Abd-Alla HI (2018) Berries supplementation modulates body weight and metabolic deteriorations in obese rats. Asian J. Pharm. Clin. Res., 11: 322-328.
Cheng K, Song Z, Zhang H, Li S, Wang C, Zhang L, et al (2019) The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression. Med. Mol. Morphol. doi: 10.1007/s00795-019-00216-7.
Caballero F, Fernandez A, De Lacy AM, Fernandez-Checa JC, Caballeria J, Garcia-Ruiz C (2009) Enhanced free cholesterol, SREBP-2 and StAR expression in human NASH. J. Hepatol. 50:789–796.
Do GM, Oh HY, Kwon EY, Cho YY, Shin SK, Park HJ, et al (2011) Long-term adaptation of global transcription and metabolism in the liver of high-fat diet-fed C57BL/6Jmice. Mol. Nutr. Food Res. 55: 173–185.
Ntambi JM, Miyazaki M, Stoehr JP, Lan H, Kendziorski CM, Yandell BS, et al (2002) Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc. Natl. Acad. Sci. USA 99: 11482–11486.
McDougall GJ, Kulkarni NN, Stewart D (2008) Current developments on the inhibitory effects of berry polyphenols on digestive enzymes. Biofactors 34:73–80.
Guttenplan JB, Chen KM, Sun YW, Lajara B, Shalaby NAE, Kosinska W, et al (2017) Effects of black raspberry extract and berry compounds on repair of DNA damage and mutagenesis induced by chemical and physical agents in human oral leukoplakia and rat oral fibroblasts. Chem. Res. Toxicol. 30:
Refbacks
- There are currently no refbacks.
