The Activity Test of Ethanol Extract in Hibiscus Leaves (Hibiscus Tiliaceus L.) as the α-Glucosidase Enzyme Inhibitor by Using ELISA (Enzyme Linked Immunosorbent Assay) Reader

Abstract

Hibiscus leaves (Hibiscus tiliaceus L.) was a plant from the family of Malvaceae, which contained triterpenoid, saponins, flavonoids, and polyphenols compounds that functioned as antidiabetic mellitus. The purpose of this study was to determine the activity of α-glucosidase enzyme inhibitor from the ethanol extract of hibiscus leaves (Hibiscus tiliaceus L.). The test was carried out through in vitro by using α-glucosidase enzyme and p-nitrofenil-α-D-glukopiranosida (PNPG) substrate, and measured by ELISA reader. The simplicia was macerated with alcohol 96%. The reaction of α-glucosidase enzyme and p-nitrofenil-α-D-glukopiranosida substrate produced yellow p-nitrofenol. The reaction product was measured at a wavelength of 405 nm. The results showed that ethanol extract of hibiscus leaves (Hibiscus tiliaceus L.) had activity as an inhibitor of α-glucosidase enzyme with Inhibitor Consentration IC50 37.98 µg / mL and could be categorized as active.

Keywords: Hibiscus tiliaceus L., Hibiscus leaves, Α-glucosidase enzyme inhibitor.