Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine

Jeanne Adiwinata Pawitan, Melisa Leviana, Dewi Sukmawati, Isabella Kurnia Liem, Ria Margiana, Twidy Tarcisia


Abstract: Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests.  We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.

Full Text:



Pawitan JA. Prospect of stem cell conditioned medium in regenerative medicine, Biomed Research International 2014;2014:965849.

Bakondi B, Shimada IS, Perry A, Munoz JR, Ylostalo J, Howard AB, et al. CD133 identifies a human bone marrow stem/progenitor cell sub-population with a repertoire of secreted factors that protect against stroke. Mol Ther. 2009;17(11):1938-47.

Mirabella T, Cilli M, Carlone S, Cancedda R, Gentili C. Amniotic liquid derived stem cells as reservoir of secreted angiogenic factors capable of stimulating neo-arteriogenesis in an ischemic model. Biomaterials 2011; 32(15): 3689–99.

Mishra PJ, Mishra PJ, Banerjee D. Cell-free derivatives from mesenchymal stem cells are effective in wound therapy. World J Stem Cells. 2012;4(5):35-43.

Bhang SH, Lee S, Shin JY, Lee TJ, Jang HK, Kim BS.Efficacious and Clinically Relevant Conditioned-medium of Human Adipose-derived Stem Cells for Therapeutic Angiogenesis. Mol Ther 2014;22(4): 862.

Chang CP, Chio CC, Cheong CU, Chao CM, Cheng BC, Lin MT. Hypoxic preconditioning enhances the therapeutic potential of the secretome from cultured human mesenchymal stem cells in experimental traumatic brain injury. Clin Sci (Lond). 2013;124(3):165-76.

Chuang TJ, Lin KC, Chio CC, Wang CC, Chang CP, Kuo JR. Effects of secretome obtained from normoxia-preconditioned human mesenchymal stem cells in traumatic brain injury rats. J Trauma Acute Care Surg. 2012;73(5):1161-7.

Pawitan JA, Liem IK, Budiyanti E, Fasha I, Feroniasanti L, Jamaan T, et al. Umbilical cord derived stem cell culture: multiple-harvest explant method. Int J PharmTech Res 2014;6(4): 1202-8.

Sari P, Luviah E, Nugraha Y, Pawitan JA, Lilianty J, Purwoko RY. Various growth factor yields in various platelet rich plasma processing methods. BTAIJ 2014; 9(11):475-8.

Nugraha Y, Sari P, Purwoko RY, Luviah E, Pawitan JA. Effect of Lysed Platelet Count in Platelet Concentrates on Various Growth Factor Levels after Freeze Thaw Cycles. Int J PharmTech Res 2014; 6(7): 2036-42.

Sari P, Luviah E, Purwoko RY, Pawitan JA. Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdatedplatelets lysate containing media. Int J PharmTech Res 2017;10(2):120-30.

Pawitan JA, Kispa T, Mediana D, Goei N, Fasha I, Liem IK, et al. Simple production method of umbilical cord derived mesenchymal stem cell using xeno-free materials for translational research. J Chem Pharm Res 2015;7(8):652-6.

Ribeiro CA, Fraga JS, Grãos M, Neves NM, Reis RL, Gimble JM, et al. The secretome of stem cells isolated from the adipose tissue and Wharton jelly acts differently on central nervous system derived cell populations. Stem Cell Research & Therapy 2012 3:18 (7 pages).

Amable PR, Teixeira MVT, Carias RBV, Granjeiro JM, Borojevic R. Mesenchymal Stromal Cell Proliferation, Gene Expression and Protein Productionin Human Platelet-Rich Plasma-Supplemented Media. PLoS ONE 2014;9(8): e104662.

Inukai T, Katagiri W, Yoshimi R, Osugi M, Kawai T, Hibi H, et al. Novel application of stem cell-derived factors for periodontal regeneration. Biochem Biophys Res Commun. 2013;430(2):763-8.

Sadat S, Gehmert S, Song YH, Yen Y, Bai X, Gaiser S, et al. The cardioprotective effect of mesenchymal stem cells is mediated by IGF-I and VEGF. Biochemical and Biophysical Research Communications 2007;363(3): 674–9.

Park BS, Kim WS, Choi JS, Kim HK, Won JH, Ohkubo F, et al. Hair growthstimulated by conditioned medium of adipose-derivedstem cells is enhanced by hypoxia: evidence ofincreased growth factor secretion. Biomed Res.2010;31:27–34.

Martin-Padura I, Gregato G, Marighetti P, Mancuso P, Calleri A, Corsini C, et al. The white adipose tissue used in lipotransferprocedures is a rich reservoir of CD34+ progenitors able to promote cancer progression. Cancer Res 2012; 72(1) :325-34.

Mitchel JB, McIntosh K, Zvonic S, Garrett S, Floyd ZE, Kloster A, et al. Immunophenotype of human adipose-derived cells: Temporal changes in stromal-associated and stem cell–associated markers. Stem Cells 2006;24:376–85.

Yoo KH, Jang IK, Lee MW, Kim HE, Yang MS, Eom Y, et al. Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues. Cell Immunol 2009; 259: 150-6.

Chan KY, Lindquist TD, Edenfield MJ, Nicolson MA, Banks AR. Pharmacokinetic Study of Recombinant Human Epidermal Growth Factor in the Anterior Eye. Investigative Ophthalmology & Visual Science1991;32 (13):3209-15.

Lee K, Silva EA, Mooney DJ. Growth factor delivery-based tissue engineering: general approaches and a review of recent developments. J R Soc Interface 2011; 8: 153–70.

Sari P, Luviah E, Nugraha Y, Pawitan JA, Lilianty J, Purwoko RY. Various growth factor yields in various platelet rich plasma processing methods. Biotechnology: An Indian Journal 2014; 9(11):475-8.

Muttukrishna S, Swer M, Suri S, Jamil A, Calleja-Agius J, Gangooly S, et al. Soluble Flt-1 and PlGF: New Markers of early pregnancy loss? PLoS ONE 2011; 6(3): e18041.

Phinney DG, Pittenger MF. Concise Review: MSC-Derived Exosomes for Cell-Free Therapy. STEM CELLS 2017;35:851–8.


  • There are currently no refbacks.