Sequencing of GTFD Gene among Biofilm Genes (GTF) in Streptococcus Mutans Isolated from Dental Caries

Abstract

Objectives: Streptococcus mutans has been implicated as a primary etiologic agent of humans dental caries. An important virulence properties of this bacterium is its ability to form biofilm known as dental plaque on tooth surfaces, these bacteria also produces glucosyltransferases, multiple glucan-binding proteins, collagen-binding protein, and protein antigen c, surface proteins that coordinate to produce dental plaque, thus inducing dental caries. This study aimed to detect gtfB, gtfC and gtfD genes in Streptococcus mutans isolated from dental caries patients in Babylon Province, Iraq. Methods: Out of 150 samples were collected from patients with dental caries35 isolates were diagnosed as Streptococcus mutans. The bacterial isolates were identified by standard laboratory protocols. Polymerase chain reaction (PCR) was used for detecting gtfB, gtfC and gtfD genes and Sequencing of gtfD genes confirmed of the results. Results: Out of, 35isolatesof Streptococcus mutans, 30(85.7%) isolates had gtfB encode GtfB with 80 bp, 27 (77.14%) had gtfC encode GtfC with 81 bp, and 31 (88.57%) had gtfD with 324 bp which explain their potential of biofilm formation. Conclusion: Our study results showed presence of High frequency of gtf genes among the clinical isolates of S. mutans isolates in Babylon Province, Iraq.