Electrochemical Determination of Tetracycline at SPCE modified with Nanocomposite

Abstract

An electrochemical sensor based on manganese dioxide nanorodMnO₂and Graphene oxide (GO) functionalized with 4-amino, 3-substituted 1H, 1, 2, 4 Triazole 5(4H) thion  (FGO)/MnO₂Nanocompositewas developed for voltammetric determination of Tetracycline (TET).The working electrode WE of SPCE was modified bya drop casting method. X-ray powder diffractometer (XRD), scanning electron microscopy (SEM) and FT-IR were employed to characterize the synthesized FGO/MnO₂.  The determination of TET at the modified electrode was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in the phosphate buffer solution (PBS).TET  show sharp increase in the oxidation peaks  in the pH 2.Voltammetric characteristics of TET (E_pa, I_pa) were estimated. From effect of scan rate, the average surface coverage ( ) of TET on the surface of  SPCE modified with FGO/MnO₂ nanocomposite, the electron transfer coefficient and electron transfer constant rate k_et was obtained as  10.56×10¹⁴ molecule cm^-2, 0.5, and 10.41 cm.s^-1respectively.  Under the optimal conditions, the peak current shows  linear relationship with concentration of Tetracycline (TET) ranges 1–9 µM with the detection limit of 0.2  M. The modified SPCE was successfully used to detect TET

Keywords: SPCE, Functionalize graphene oxide, Cyclic voltammetry.