The Evaluation of IL-4, IL-6 and Histopathology Study in Mice Infected with Entamoeba histolytica and Treated with Proteins Extracted from Shrimp

Abstract

Approximately 60% of the world’s population is infected with intestinal parasites. Entamoeba histolytica constitutes one of the commonest protozoal infections in world, and it is estimated to infect 50 million cases of invasive amebiasis, and about 100,000 deaths and probably represent the third leading parasitic cause of death, behind only malaria and schistosomiasis. Oral inoculation of mice with (1*10³ cell/0.1ml) cysts of E. histolytica isolated from the feces of 170 patients with amoebasis in Ibn Al-balady hospital, AL-Kindi Teaching hospital and Fatima Al-Zahraa hospital in Iraq resulted in an infection between (2-3) days after inoculation with the parasite .Among all the infected mice as shown by the presence of trophozoites in the large intestine and colon. Many histological abnormalities in the large intestine and the liver were detected in experimentally infected mice with Entamoeba histolytica. These abnormalities as necrosis, severe hemorrhage, increased in numbers of goblet cells, congested blood vessels. High level of IL-6 and IL-4 in the positive group compare with the negative group and the groups that treated with flagel and the group that treat with shrimp extracted proteins.

Keywords: Entamoeba histolytica, Shrimp extracted proteins, IL-4, IL-6, Histopathology study.