The Relationship between Phylogenic Typing and Antimicrobial Susceptibility Patterns Forescherichia Coliisolatedfrom Utis in Many Hospitals at Baghdad City

Abstract

Objective In the currentsearch , E.coli  isolated from UTIs in many Baghdad hospitals. The study concentrated on phylogenic groups and this was done based on triplex PCR method by primers besieged to three genetic markers, chuA, yjaA and TspE4.C2. Evaluate the relationship of phylogenic groups of E.coli isolates with the antibiotic-non sensitive patterns.Methodology Four hundredof E.coli bacteria inaccessible from urine samples from  five hospitals in Baghdad city includes : Ghazi AL-Hariri, Iben- Beledi , AL-Iskan , AL-Nooman and AL-Yarmoke hospitals. Phylogenetic categorization of E. coli isolates was completed by by means ofearlier reported triplex PCR-based on  phylotyping procedure using primers besieged at three markers, chuA, yjaAand TspE4.C2. Phylogenetic combination was done on the starting point of the existence or nonexistence of the3 DNA fragments as follows: chuA–, TspE4.C2–, group A; chuA–, yjaA–, TspE4.C2+, group B1; chuA+, yjaA+, group B2; chuA+, yjaA–, group D. Because two possible profiles can be obtained for the groups A, B2, and D. Fifteen antibiotics second-hand for all foremost groups and their individual generations were used in this paper adjacent to all bacterial isolates. Results The isolates ofE.colifrom UTI  were distributed within thephylogroups B2 (43%); phylogroups D (28.25%); phylogroups A (24%) and B1 (3.5%), so phylogenic group B1 was prevalent among  male patients of AL-Yermouk hospital ( 14%), followed AL-Iskan (3.75)% so, isolation rate of E.coli were higher among age (11-20 ) years (31.75 %), also(11%) were sensitivity to antibiotics, whilst (75.25%) were classified as extensive drug resistance pathogens, but  13.75% were multidrug resistance  and in Qarkh areas, the frequency of phylogeneticB2 was (59.88%), followed phylogenetic A and D were ( 68.75 and 48.78%) respectively as  compared to Rusafa areas were prevalence phylogenetic B₂(40.12%),followedphylogenetic D and A were (51.22 and 31.25 %) , as well as most of these phylogenetic groups occurred more frequently in both groups B2 or D (43, 28.25) % respectively, also ( 2.75, 2.5 )% of phylogenic A were antibiotics sensitivity in both AL-Iskan hospital and Ghazi AL-Hariri hospital respectively whilst these phylogenic was multidrug resistance as percentage (9, 3.75) % respectively in both AL-Yermouk hospital and AL-Iskan hospital, Whilst in these hospital this phylogenic A was founded with extensive drug resistance (1, 0.75) % respectively, also most of phylogenetic group  B2 occurred more frequently in AL-Yermouk hospital, so the sensitivity; multidrug resistance and extensive drug resistance as (2.25, 15 and 3.5) % respectively, also all  phylogenetic groups E. coli isolates from UTI patients of five hospital weregrouped  in two or three major group as  A , B group contain major sub groups .RecommendationsThe study recommends additional studies of the correlation  between the Phylogenic group  with antibiotic-resistance pattern  for other bacterial  types  isolated from  UTIs or the same bacteria isolated from other types of infections. We suggest to applicate our study on bacteria Vibrio colerai  isolated from the infected patients stool who submitted to  the same hospitals  or in the other provinces of the country, because of our country suffering frome  epidemics infections  in the last two years.

 

Keywords: Urinary tract infections, Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), E. coli, Antibiotic resistance, MDR.