Anti-obesity effect of Dunaliella salina: Study on the gastric inhibitory polypeptide, ROS, DNA damage and metabolic gene expression in rats
Abstract
This study aimed to identify the gene expression profiling of lipid related genes in animal model of obesity and the role of microalgal Dunaliella salina (alga) in modulating the expression of these genes during the initial period of obesity. The diet-induced obesity model was used using fifty male Wistar albino rats (weighing 150-160 g) that have obesity caused by being fed high-fat diet (HFD) for 12 weeks. These rats were randomly divided into 2 groups (10 rats each) and treated with D. salina extract and orlistat for 6 weeks. The anti-obesity effect of this halophilic unicellular green alga was compared to orlistat, a standard anti-obesity drug promoting weight loss as a gastric and pancreatic lipase inhibitor. Treatment with the alga and orlistat showed significant effect in reducing gastric inhibitory polypeptide (GIP). Also, the treatment of obesity-induced rats with the alga increased significantly the mRNA expression levels of lipid catabolic genes (peroxisome proliferator-activated receptor PPAR-α and carnitine palmitoyltransferase CPT-1) and decreased significantly the expression levels of lipid anabolic gene (SCD-1) compared with obesity-induced rats. In addition, D. salina extract was able to decrease ROS generation and inhibit the rate of DNA damage in obese rats. The study was extended to evaluate the phytochemical composition of microalgal extract. The fatty acid methyl esters were analyzed by GLC. The extract contained considerable level of omega-3 fatty acids. The presence of palmitic (62.5%), linolenic (9%), oleic (8.6%) and linoleic (7.4%) was revealed. The percentage of saturated and unsaturated fatty acids were 67.7 and 32.1%, respectively. D. salina extract administration which is rich in omega-3 fatty acids, has anti-obesity properties. Results suggested that D. salina might be available as functional alga and bioactive diet supplement for the treatment of obesity.
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Cowie CC, Rust KF, Byrd-Holt DD, Eberhardt MS, Flegal KM, Engelgau MM, et al (2006) Prevalence of diabetes and impaired fasting glucose in adults in the U.S. population: National health and nutrition examination survey 1999–2002. Diabetes Care, 29:1263–1268.
Ogden CL, Carroll MD, Curtin LR, McDowell MA, Tabak CJ, Flegal KM (2006) Prevalence of overweight and obesity in the United States, 1999–2004. JAMA, 295:1549–1555.
Shibue K, Yamane S, Harada N, Hamasaki A, Suzuki K, Joo E, Iwasaki K, et al (2015). Fatty acid binding protein 5 regulates diet-induced obesity via GIP secretion from enteroendocrine K cells in response to fatingestion. Am. J. Physiol. Endocrinol. Metab., 308:E583–91.
Rajala MW, Scherer PE (2003) Minireview: The adipocyte–at the crossroads of energy homeostasis, inflammation, and atherosclerosis. Endocrinology, 144: 3765–3773.
Trayhurn P (2005) Endocrine and signalling role of adipose tissue: new perspectives on fat. Acta Physiol. Scand., 184:285–293.
Tartare-Deckert S, Chavey C, Monthousel MN, Gautier N, Van Obberghen E (2001)The matricellular protein SPARC/osteonectin, as a newly identified factor upregulated in obesity. J. Biol. Chem., 276:22231–22237.
Nadler ST, Stoehr JP, Schueler KL, Tanimoto G, Yandell BS, Atti AD (2000) The expression of adipogenic genes is decreased in obesity and diabetes mellitus. Proc. Natl. Acad. Sci. USA, 97: 11371–11376.
Aguilar EC, Jascolka TL, Teixeira LG, Lages PC, Ribeiro AC, Vieira EL, et al (2012) Paradoxical effect of a Pequi oil-rich diet on the development of atherosclerosis: balance between antioxidant and hyperlipidemic properties. Braz. J. Med. Biol. Res., 45: 601–609.
Ye ZW, Jiang JG, Wu GH (2008) Biosynthesis and regulation of carotenoids in Dunaliella: progresses and prospects. Biotechnol. Adv., 26:352–360.
Sathasivam R, Ki JS (2018) A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Mar. Drugs, 16. pii: E26. doi: 10.3390/md16010026.
Ahmed HH, Hegazi MM, Abd-Alla HI, Eskander EF, Ellithey MS (2011) Antitumour and antioxidant activity of some Red Sea seaweeds in Ehrlich ascites carcinoma in vivo. Z Naturforsch C, 66:367-376.
Stanier RY, Kunisawa MM, Cohn-Bazire G (1971) Purification and properties of unicellular blue green algae (order Chroococcales). Bacteriol. Rev., 35:171-205.
Adaramoye O, Akinatyo O, Achen J, Michel A (2008) Lipid lowering effects of methanolic extracts of Vernonia anygdalina leaves in rats fed on high cholesterol diet. Vasc. Health Risk Manag., 4: 235-241.
El-BazF K, Aly HF, Abd-Alla HI, Fayed DB (2018) Therapeutic impact of berries (Morus alba and Morus rubra) fruit extract in the regression of high-fat diet-induced cardiac dysfunction in rats. Asian J. Pharm. Clin. Res., 11; 314-320.
Shalaby HM, Tawfek NS, Abo-Elhussein BK, Abd El-Ghany MS (2015) The assessment of some biochemical and immunological effects by amphetamine and orlistat on obesity in rats. Food Public Health, 4:185-192.
Rupérez FJ, GarcÃa-MartÃnez D, Baena B, Maeso N, Cifuentes A, Barbas C, et al (2008) Evolution of oxidative stress parameters and response to oral vitamins E and C in streptozotocin-induced diabetic rats. J. Pharm. Pharmacol., 60:871-878.
Selek S, Aslan M, Nazlıgul Y (2012) Serum PON1 activity and oxidative stress in non-alcoholic fatty liver disease. J. Harran Univ. Med. Fac., 9: 85-91.
Morgan LM, Morris BA, Mark V (1978) Radioimmunoassay of gastric inhibitory polypeptide. Ann. Clin. Biochem., 15;172-177.
Cetica PD, Pintos LN, Dalvit GC, Beconi MT (2001) Antioxidant enzyme activity and oxidative stress in bovine oocyte in vitro maturation. IUBMB Life, 51: 57-64.
El-Baz FK, Khalil WK, Booles HF, Aly HF, Ali GH (2016) Dunaliella salina suppress oxidative stress, alterations in the expression of pro-apoptosis and inflammation related genes induced by STZ in diabetic rats. Int. J. Pharm. Sci. Rev. Res., 38:219-226.
Yook JS, Kim KA, Park JE, Lee SH, Cha YS (2015) Microalgal oil supplementation has an anti-obesity effect in C57BL/6J mice fed a high fat diet. Prev. Nutr. Food Sci., 20:230-7.
Khalil WK, Booles HF (2011) Protective role of selenium against over-expression of cancer-related apoptotic genes induced by O-cresol in rats. Arh. Hig. Rada. Toksikol., 62:121-129.
Linjawi SA, Khalil WK, Salem LM (2014) Detoxified Jatropha curcas kernel meal impact against benzene-induced genetic toxicity in male rats. Int. J. Pharm., 4:57-66.
Fairbairn DW, Olive PL, O’Neill KL (1995) Comet assay: A comprehensive review. Mutat. Res., 339: 37-59.
Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, et al (1997) Comet assay in human biomonitoring studies: Reliability, validation, and applications. Environ. Mol. Mutagen., 30:139-146.
Johnson AR, Davenport JB (1971) Biochemistry and Methodology of Lipids. New York: John Wiley and Sons, Inc31.
Song DH, Getty-Kaushik L, Tseng E, Simon J, Corkey BE, Wolfe MM (2007) Glucose dependent insulinotropic polypeptide enhances adipocyte development and glucose uptake in part through Akt activation. Gastroenterol., 133:1796–805.
Nasteska D, Harada N, Suzuki K, Yamane S, Hamasaki A, Joo E, et al (2014) Chronic reduction of GIP secretion alleviates obesity and insulin resistance under high-fat diet conditions. Diabetes, 63: 2332–2343.
Bailey CJ, Flatt PR, Kwasowski P, Powell CJ, Marks V (1986) Immunoreactive gastric inhibitory polypeptide and K cell hyperplasia in obese hyperglycaemic (ob/ob) mice fed high fat and high carbohydrate cafeteria diets. Acta Endocrinol. (Copenh), 112:224–229.
Usdin TB, Mezey E, Button DC, Brownstein MJ, Bonner TI (1993) Gastric inhibitory polypeptide receptor, a member of the secretin-vasoactive intestinal peptide receptor family, is widely distributed in peripheral organs and the brain. Endocrinology, 133:2861–2870.
Naitoh R, Miyawaki K, Harada N, Mizunoya W, Toyoda K, Fushiki T, et al (2008) Inhibition of GIP signaling modulates adiponectin levels under high-fat diet in mice. Biochem. Biophys. Res. Commun., 376: 21–25.
Harada N, Hamasaki A, Yamane S, Muraoka A, Joo E, Fujita K, et al (2011) Plasma gastric inhibitory polypeptide and glucagon-like peptide-1 levels after glucose loading are associated with different factors in Japanese subjects. J. Diabetes Investig., 2:193–199.
Brøns C, Jensen CB, Storgaard H, Hiscock NJ, White A, Appel JS, , et al (2009) Impact of short-term high-fat feeding on glucose and insulin metabolism in young healthy men. J. Physiol., 587:2387–2397.
Simopoulos AP (2008)The importance of the omega-6/omega- 3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp. Biol. Med., 233:674-688.
Fiamoncini J, Turner N, Hirabara SM, Salgado TM, Marçal AC, Leslie S, et al (2013) Enhanced peroxisomal ï¢-oxidation is associated with prevention of obesity and glucose intolerance by fish oil-enriched diets. Obesity, 21:1200-1207.
Rasmy GE, Khalil WK, Moharib SA, Ahmed KA, Jwanny EE (2011) Dietary fish oil modulates the effect of dimethylhydrazine-induced colon cancer in rats. Grasas y. Aceites, 62: 253-267.
Azain MJ(2004) Role of fatty acids in adipocyte growth and development. J. Anim. Sci., 82: 916-924.
Ruzickova J, Rossmeisl M, Prazak T, Flachs P, Sponarova J, Veck M, et al (2004) Omega-3 PUFA of marine origin limit diet-induced obesity in mice by reducing cellularity of adipose tissue. Lipids, 39:1177-1185.
Chitranjali T, Anoop Chandran P, Muraleedhara Kurup G (2015) Omega-3 fatty acid concentrate from Dunaliella salina possesses anti-inflammatory properties including blockade of NF-κB nuclear translocation. Immunopharmacol. Immunotoxicol., 37: 81-89.
Rao AR, Sarada R, Shylaja MD, Ravishankar GA (2015) Evaluation of hepatoprotective and antioxidant activity of astaxanthin and astaxanthin esters from microalga-Haematococcus pluvialis. J. Food Sci. Technol., 52: 6703-6710.
Perkins ND. Integrating cell-signalling pathways with NF-kappa B and IKK function. Nat. Rev. Mol. Cell Biol., 8: 49-62.
Xue KX, Wu JZ, Ma GJ, Yuan S, Qin HL (1998) Comparative studies on genotoxicity and antigenotoxicity of natural and synthetic β-carotene stereoisomers. Mutation Res., 418: 73-78.
Jump DB (2008) N-3 polyunsaturated fatty acid regulation of hepatic gene transcription. Curr. Opin. Lipidol., 19:242-247.
Sampath H, Ntambi JM (2004) Polyunsaturated fatty acid regulation of gene expression. Nutr. Rev., 62:333-339.
McGarry JD, Leatherman GF, Foster DW (1978) Carnitine palmitoyl transferase I−the site of inhibition of hepatic fatty acid oxidation by malonyl-CoA. J. Biol. Chem., 253:4128-4136.
Miyazawa S, Hayashi H, Hijikata M, Ishii N, Furuta S, Kagamiyama H, et al., (1987) Complete nucleotide sequence of cDNA and predicted amino acid sequence of rat acyl-CoA oxidase. J. Biol. Chem., 262:8131-8137.
Gong F, Wang J, Li J. (2017) Isolation and characterization of peroxiredoxin 1 gene of Dunaliella salina. Gene, 635: 39-45.
Yee JK, Wahjudi PN, Vega J, Lim S, Martin A, Patterson ME, et al. (2013) Stearoyl-CoA desaturase enzyme 1 inhibition reduces glucose utilization for de novo fatty acid synthesis and cell proliferation in 3T3-L1 adipocytes. Metabolomics, 9:809-816.
Lu RH, Liang XF, Wang M, Zhou Y, Bai XL, He Y (2012) The role of leptin in lipid metabolism in fatty degenerated hepatocytes of the grass carp Ctenopharyngodonidellus. Fish Physiol. Biochem., 38:1759-1774.
FernándezGianotti T, Burgueño A, Gonzales Mansilla N, Pirola CJ, Sookoian S (2013) Fatty liver is associated with transcriptional downregulation of stearoyl-CoA desaturase and impaired protein dimerization. PLoS One 8:e76
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