In-vitro Antioxidant and Cytotoxic Activities of Silver Nanoparticles of Mangiferin Isolated from Mangifera indica

Mahendran Sekar


The present study aimed to synthesize mangiferin AgNPs and evaluate its in vitro antioxidant and cytotoxic properties. Mangiferin AgNPs was prepared using silver nitrate solution and characterized by SEM and Zeta potential measurement. In-vitro antioxidant and cytotoxic studies of mangiferin and its AgNPs were conducted by using DPPH and MTT assay method, respectively. The synthesized mangiferin AgNPs with particle size ranges from 25.30 - 40.79 nm. The charge of mangiferin AgNPs were confirmed by Zeta potential and found to be −33.7 mV. Mangiferin AgNPs showed 90.43 ± 1.90 % of inhibition at 500 µg/ml and the activity was well comparable with mangiferin in DPPH method. Mangiferin AgNPs exhibited significant reduction in cancerous cell growth when compared to Mangiferin in all the tested concentrations ranges from 10-100 µg/ml. This results representing that the mangiferin AgNPs was a capable nanocarrrier for distributing mangiferin to cancer cells. In future, mangifrin AgNPs will be further tested with other cancer cell lines to confirm its safety and effectiveness.

Full Text:



Muruganandan S, Gupta S, Kataria M, Lal J, Gupta PK (2002) Mangiferin protects the streptozotocin induced oxidative damage to cardiac and renal tissues in rats. Toxicology 176:165–173.

Rajendran P, Ekambaram G, Sakthisekaran D (2008) Protective role of mangiferin against Benzo (a) pyrene induced lung carcinogenesis in experimental animals. Biol Pharm Bull 31:1053–1058.

Leiro JM, Alvarez E, Arranz JA, Siso IG, Orallo F (2003) In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor–alpha and transforming growth factor–beta genes. Biochem Pharmacol 65:1361–1371.

Andreu GP, Delgado R, Velho JA, Curti C, Vercesi AE (2005) Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe2+–citrate. Eur J Pharmacol 513:47–55.

Garcia D, Delgado R, Ubeira FM, Leiro J (2002) Modulation of rat macrophage function by the Mangifera indica L. extracts Vimang and mangiferin. Int Immunopharmacol 2:797–806.

. Zhu XM, Song JX, Huang ZZ, Wu YM, Yu MJ (1993) Antiviral activity of mangiferin against herpes simplex virus type 2 In vitro. Zhongguo Yao Li XueBao 14:452–454.

Bairy I, Reeja S, Siddharth, Rao PS, Bhat M, Shivananda PG (2002) Evaluation of antibacterial activity of Mangifera indica on anaerobic dental microglora based on in vivo studies. Indian J Pathol Microbiol 45:307–310.

Mahendran S, Badami S, Ravi S, Thippeswamy BS, Veerapur VP (2014) Synthesis and Evaluation of Analgesic and Anti-Inflammatory Activities of Most Active Free Radical Scavenging Derivatives of Mangiferin. British J Applied Sci Tech 4:4959-4973.

Mahendran, S (2014) Molecules of Interest – Mangiferin - A Review. Annual Res Rev Biol 5:307-320.

Dongre SH, Badami S, Ashok G, Mahendran S, Vijayan P, Suresh B (2008) Cytotoxic and Antioxidant Properties of Four Plants Belonging to the genus solanum. Oriental Pharmacy and Exp Med 8:86-92.

Yoshimia N, Matsunagaa K, Katayamaa M, Yamadaa Y, Kunoa T, Qiaoa Z, et al., (2001) The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats. Cancer Lett 163:163-70.


  • There are currently no refbacks.