Mathematical Design of the Experiment in the Development of Technology of Beeswax obtaining from Propolis

Oleg Shpychak


In present material the development of the technology of purified beeswax obtaining from propolis, which meets modern physical-chemical and therapeutic requirements, using a various models of mathematical design of the experiment has been shown.

In this work a petroleum-ether fraction of propolis containing a beeswax products was used. While analyzing of the obtained fraction, lipids and some other high-molecular compounds of unqualified structure are extracted in parallel, as well as an insignificant number of the γ-pyrone derivatives.

In this connection, the development of the condition of beeswax obtaining from this fraction, which is maximally purified from the related substances was reasonable. For this purpose a series of classical methods of fractionally differentiated extraction followed by fractions evaporation in a vacuum was used. In addition, methods of precipitation using a some organic solvents were used.

The optimal conditions of the technology of purified beeswax obtaining from propolis have been determined by methods of mathematical design.

Keywords: propolis, beeswax, technology, methods of fractionally differentiated extraction, precipitation, methods of mathematical design.

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Pertsev IM, Dmitryevsky DI, Ribachuk VD et al. Auxiliary substances in drug technology: impact on technological, consumer, economic and therapeutic efficacy. Kharkiv; 2010. 600 p.

Derzhavnii reєstr lіkars'kih zasobіv Ukraїni [Elektronnii resurs]. MOZ Ukraїni; golov. red. Sharikіna NІ. K.: PC WORLD UKRAINE; 2018. Available from:

Tikhonov AI., Bogdan NS., Shpychak OS. Perspective towards the creation Alternative medical maintenance the population of extemporaneous preparations of bee productsю Collection of scientific works of staff members of NMAPE, Kyiv, 2014;23(3):429-433. – Available from:

Fratini F, Cilia G, Turchi B, Felicioli A. Beeswax: A minireview of its antimicrobial activity and its application in medicine. Asian Pacific Journal of Tropical Medicine 2016;9(Issue 9):839–843. – Available from:

Giampieri F, Quiles JL., Orantes-Bermejo FJ., Gasparrini M, Forbes-Hernandez TY., Sánchez-González C. et al. Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties? Food and Chemical Toxicology 2018;112:126–133. – Available from:

Soleimanian Y, Goli SAH, Varshosaz J, Sahafi SM. Formulation and characterization of novel nanostructured lipid carriers made from beeswax, propolis wax and pomegranate seed oil. Food Chemistry 2018;244:83–92. – Available from:

Tichonov AI, Jarnych TG, Czernych WP et al. 2005 Teoria I praktyka wytwarzania leczniczych preparatow propolisowych. Khrakov: Drukaznia «Marka». 274 c.

Uner M, Gönüllü U, Yener G, Altinkurt T. A new approach for preparing a controlled release ketoprofen tablets by using beeswax. Farmaco 2005;60(Issue 1):27-31. – Available from:

Garai L. Improving HPLC Analysis of Vitamin A and E: Use of Statistical Experimental Design. Procedia Computer Science 2017;108:1500-1511. – Available from:

Teixeira KSS, Fonseca SGC, De Moura LCB, De Moura MLR, Borges MHP, Barbosa EG, De Lima e Moura TFA. Use of chemometrics to compare NIR and HPLC for the simultaneous determination of drug levels in fixed-dose combination tablets employed in tuberculosis treatment. Journal of Pharmaceutical and Biomedical Analysis 2018;149(5):557-563. – Available from:

Kovács A, Berkó Sz, Csányi E, Csóka I. Development of nanostructured lipid carriers containing salicyclic acid for dermal use based on the Quality by Design method. European Journal of Pharmaceutical Sciences 2017;99:246-257. – Available from:

Tucaković D Barišić, Grahek Ž. Application of mathematical calibrations in measurements of gamma emitting radionuclides in honey samples and borosilicate filters. Applied Radiation and Isotopes 2017;126:162-164. –Available from:

Oroian M, Ropciuc S. Honey authentication based on physicochemical parameters and phenolic compounds. Computers and Electronics in Agriculture 2017;138:148-156. – Available from:

Ansari MJ, Al-Ghamdi A, Khan KA, Adgaba N, El-Ahmady SH, Gad HA, Roshan A, Meo SA, Kolyali S. Validation of botanical origins and geographical sources of some Saudi honeys using ultraviolet spectroscopy and chemometric analysis. Saudi Journal of Biological Sciences 2018; 25(Issue 2):377-382. –Available from:

Pukelsheim F. Optimal Design of Experiments. Society for Industrial and Applied Mathematics 2006. 479 р. – Available from: https://doi:10.1137/1.9780898719109.

Grachev YuP, Plaksin YM, Matematicheskiye metody planirovaniya eksperimenta (Mathematical Methods of Experiment Planning). Moscow: De Li Print; 2005.

Prochaska Ch, Theodore L. Introduction to Mathematical Methods for Environmental Engineers and Scientists. John Wiley & Sons; 2018. 498 p.

Montgomery DC. Statistical Quality Control, 7th Edition. Kindle Edition; 2012. 768 p.

Eriksson L, Johansson E, Kettaneh-Wold N, Wikstrцm C, Wold S. Design of Experiments: Principles and Application. Umetrics Academy, 2008. pp. 1-425.

Yan X, Gang Su X. Theory and Computing: Linear Regression Analysis. 2009. pp.1-348.

:TIBCO Statistica™: Quick Reference Software Release 13.3. June 2017 (ElectronicVersion), 205 p.

Halafyan АА, Borovikov VP, Kalaidina GV. Teoriya veroyatnostei, matematicheskaya statistika i analiz dannih. Osnovi teorii i praktika na komp'yutere. Statistica. Excel: uchebnoe posobie. Моsкvа : URSS, cop.; 2016. 317 p.

Halafyan А. А. Promishlennaya statistika: Kontrol' kachestva, analiz protsessov, planirovanie eksperimentov v pakete STATISTICA. Izdatel'stvo: Librokom; 2013. 384 p.

Rowe P. Essential Statistics for the Pharmaceutical Sciences. Second Edition. John Wiley & Sons, Ltd. Southern Gate, Chichester, West Sussex, PO19 8SQ, UK. 2016. 409 p.

Schmuller J. Statistical Analysis with Excel For Dummies, 4th Edition. Publisher: For Dummies; 2016. 552 p.


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